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In Vitro functional experiments of GDI1 . (A). Expression differences of GDI1 among the NCM460, HCT116, and HT29 cell lines; (B)Verification of siRNA efficiency in knocking down GDI1 ; (C) Wound healing assay following GDI1 knockdown; <t>(D)</t> <t>CCK-8</t> assay after GDI1 knockdown; (E) Apoptosis assay in the HT29 cell line after GDI1 knockdown; (F) Apoptosis assay in the HCT116 cell line after GDI1 knockdown. * P < 0.05, ** P < 0.01, *** P < 0.001.
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In Vitro functional experiments of GDI1 . (A). Expression differences of GDI1 among the NCM460, HCT116, and HT29 cell lines; (B)Verification of siRNA efficiency in knocking down GDI1 ; (C) Wound healing assay following GDI1 knockdown; <t>(D)</t> <t>CCK-8</t> assay after GDI1 knockdown; (E) Apoptosis assay in the HT29 cell line after GDI1 knockdown; (F) Apoptosis assay in the HCT116 cell line after GDI1 knockdown. * P < 0.05, ** P < 0.01, *** P < 0.001.
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In Vitro functional experiments of GDI1 . (A). Expression differences of GDI1 among the NCM460, HCT116, and HT29 cell lines; (B)Verification of siRNA efficiency in knocking down GDI1 ; (C) Wound healing assay following GDI1 knockdown; <t>(D)</t> <t>CCK-8</t> assay after GDI1 knockdown; (E) Apoptosis assay in the HT29 cell line after GDI1 knockdown; (F) Apoptosis assay in the HCT116 cell line after GDI1 knockdown. * P < 0.05, ** P < 0.01, *** P < 0.001.
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In Vitro functional experiments of GDI1 . (A). Expression differences of GDI1 among the NCM460, HCT116, and HT29 cell lines; (B)Verification of siRNA efficiency in knocking down GDI1 ; (C) Wound healing assay following GDI1 knockdown; (D) CCK-8 assay after GDI1 knockdown; (E) Apoptosis assay in the HT29 cell line after GDI1 knockdown; (F) Apoptosis assay in the HCT116 cell line after GDI1 knockdown. * P < 0.05, ** P < 0.01, *** P < 0.001.

Journal: Translational Oncology

Article Title: Construction and validation of golgi apparatus-related genes as predictors of the immune microenvironment and prognosis in colorectal cancer

doi: 10.1016/j.tranon.2026.102714

Figure Lengend Snippet: In Vitro functional experiments of GDI1 . (A). Expression differences of GDI1 among the NCM460, HCT116, and HT29 cell lines; (B)Verification of siRNA efficiency in knocking down GDI1 ; (C) Wound healing assay following GDI1 knockdown; (D) CCK-8 assay after GDI1 knockdown; (E) Apoptosis assay in the HT29 cell line after GDI1 knockdown; (F) Apoptosis assay in the HCT116 cell line after GDI1 knockdown. * P < 0.05, ** P < 0.01, *** P < 0.001.

Article Snippet: A suspension of 5000 transfected cells in 100 μL was distributed into each well of a 96-well plate, accompanied by the addition of 10 μL of CCK-8 solution (MCE, HY-K0301).

Techniques: In Vitro, Functional Assay, Expressing, Wound Healing Assay, Knockdown, CCK-8 Assay, Apoptosis Assay